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UV-FIAS is a stand-alone software package to control the PerkinElmer Flow Injection Analysis system consisting of a Lambda UV/Vis Spectrometer, a FIAS 300 module, with peristaltic pumps for sample and carrier stream and with an injection valve and a AS90 Autosampler.
The TechniqueFlow Injection Analysis (FIA) is a technique for total automation of colorimetric quantitative measurement, mainly used in water- and environmental analysis. For Flow Injection Analysis a UV/Vis spectrometer, a pump/injection module In Flow Injection Analysis the sample is injected into a continuous liquid carrier stream and the derivatisation reagents are added automatically and successively all in small Teflon tubing. The chemical reactions occur under defined conditions in the manifold. The manifold consists of a chemifold where reagent- and samples streams are combined, one or more reaction coils for the chemical derivatisation and a flow cell for detection of the coloured product. The whole process of automated on-line derivatisation takes only about 30 to 60 seconds, thus making this an ideal technique for all laboratories requiring high sample throughput for single component analysis. Typical parameters which are easy to measure with FIA are: Phosphate, Nitrate, Nitrite and Ammonium. These parameters are approved by European Norm to be measured with FIA (for Example DINE 26, Ammonium Determination). But also methods exist for the determination of many other anions and also cations and bulk parameters. The Lambda Series UV/VIS SpectrometersThe Lambda Series UV/Vis Spectrometers consist of Lambda 25, Lambda 35 and Lambda 45. All are a scanning double beam UV/Vis Spectrometer covering the wavelength range between 190nm and 1100nm. Lambda 25 is a fixed 1nm resolution instrument. Lambda 45 allows selection between four different resolutions: 0.5nm, 1nm, 2nm and 4nm resolution. The Lambda 45 includes a Pre-Monochromator that reduces The optical properties of The Lambda Series spectrometers are superior to those of the simple photometers most often used for FIA. For example a maximum drift of 0.00015 A per hour means dependable constant measurements during the time of analysis of all samples. Negligible stray light allows you to make linear measurements up to a greater absorbance, whereby the measurable concentration range is greatly extended. Negligible noise and high photometric accuracy are the basis for correct and reproducible measurements, even over long periods. Because of its big sample compartment it is possible to use long pathlength cells which increases sensitivity by a factor of 5, thus lowering the detection limit significantly. This is only one of the many unique features of the Lambda FIA - System from PerkinElmer, using a standard laboratory Spectrometer as a detector in Flow Injection Analysis. The FIAS-300 ModuleProven peristaltic pumps are the key elements in the FIAS-300 module. They can be controlled independently to supply carrier and reagents for optimum reaction conditions. The special injection valve provides reproducible and accurate sampling time after time. Pump speeds and fill/inject positions can be selected and defined individually within the UV FIAS software and allow maximum flexibility in the definition of FIA conditions. The AS-90 Autosampler
The Autosampler is also controlled by UV FIAS Software. You can enter the position of references, blank and samples positions easily on the graphical sample tray page of UV FIAS Software package.
The UV FIAS SoftwareUV FIAS Software integrates and controls the entire system. Within a FIAS Method the sample and reference positions in the Autosampler, pump speeds and timing for injection are selected. Approximately 80 optimized FIA methods UV Fias Software provides speed of analysis and ease-of use. UV Fias provides powerful peak recognition and quantitation, baseline correction and bubble detection features. The user certainly will find UV FIAS software easy to operate because it uses well known Windows operating standards. The opposite figure shows the Method-Setup page for the determination if Nitrite. This is one page of the pre-programmed application methods where pump- and injection- timing is defined, together with measurement wavelength, calibration conditions, replicates and other important parameters.
Furthermore it is possible to reload a completed measurement. Then all spectra can be displayed together in a 3-D data set, reports can be generated and printed, outliers can be removed from the calibration curve or data evaluation parameters can be modified. All modifications on data are protocolled however and can always be undone.
UV FIAS software includes a range of unique features like:
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with chemifold and an Autosampler is required together with an appropriate control- and data evaluation software.
the stray light by a factor 2. These different instrument options thus can be used for a wide range of applications and serve as a base for the application systems.
The flexible and proven AS-90 Autosampler is an integral part of the Lambda FIA - System. The AS-90 Autosampler ensures the automatic change of the sample. A range of different sample trays are available for the continuous sampling of up to 153 samples. For very special applications, the tray configurations can be use-customized.
for the most common parameters come standard with the UV FIAS software package. Their chemistry and methodology is documented in the Lambda FIA cookbook.
During a actual FIA run the UV FIAS software continuously displays the actual signal, the calibration curve and the result (raw data and calculated concentrations) of the measurement on the UV FIAS Result page. It is also possible to re-load each spectrum and display it, by just clicking on the corresponding line in the results grid.
